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Cornell University Genetically Engineered Machines
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Qiagen Miniprep
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Double Digest
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Site Directed Mutagenesis
T4 Ligase Ligation
Gibson Assembly
PCR Methods
Q5 PCR
Phusion PCR
Taq PCR
Vent PCR
Plasmid Insertion
Electroporation of
E.coli
Preparing Electrocompetent
E.coli
DNA Assessment
DNA Gel Electrophoresis
Preparation of DNA for Sequencing
Fluorescence Assay
Fungal Transformation
Preparation of Protoplasts
Transformation of Protoplasts