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Team:DTU-Denmark/Protein Models
From 2013.igem.org
Protein Models
It turns out that the structures of some of the proteins we want our mutants to express have been determined.
Contents |
Mutant 1
AMO
Ammonia monooxygenase
homology models of all three subunits of AMO
At the time of writing neither of the the three subunits from Nitrosomonas Europae is available as 3D structures, so each of the subunits was identified at UNIPROT, and their sequence was homologymodelled using [http://www.cbs.dtu.dk/services/CPHmodels/ CPHmodels-3.2]
| Resource | AmoA | AmoB | AmoC |
|---|---|---|---|
| Uniprot | [http://www.uniprot.org/uniprot/Q04507 Q04507] | [http://www.uniprot.org/uniprot/Q04508 Q04508] | [http://www.uniprot.org/uniprot/Q82T63 Q82T63] |
| Template pdb code and chain | [http://www.pdb.org/pdb/explore/explore.do?structureId=1yew 1YEW.A] | [http://www.pdb.org/pdb/explore/explore.do?structureId=1yew 1YEW.B] | [http://www.pdb.org/pdb/explore/explore.do?structureId=3rgb 3RGB.C] |
| Coverage | 90.5 | 86.2 | 66.4 |
| E-value | 1e-87 | 3e-67 | 8e-43 |
The three subunits were combined and aligned to the subunits of the [http://www.pdb.org/pdb/explore/explore.do?structureId=1yew Crystal structure] of particulate methane monooxygenase, hence an overlap of two alpha helices.
Hao
Hydroxylamine oxidoreductase
source [http://www.rcsb.org/pdb/explore.do?structureId=1fgj 1FGJ]
cc554
Cytochrome c554
source [http://www.rcsb.org/pdb/explore.do?structureId=1ft5 1FT5]
Mutant 2
NirS
Nitrite reductase
As a dimer with N2 - source [http://www.rcsb.org/pdb/explore/explore.do?structureId=1nno 1NNO]
All models were made and visualized in [http://sourceforge.net/projects/pymol/ PyMOL v1.6]
