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{
"date" : "2013-08-07",
"author" : "emil",
"title" : "Test for integration in B. subtilis ",
"content" : "In order to demonstrate the integration of pXyl in Thr locus (essential for the biosinthesis of threonine) I have prepared 40 ml of a minimal medium (MNGE) following the LMU munich protocol without the threonine.Then I did an inoculum from the plate obtained from the transformation of 29/7.
MNGE medium| Compound | Amount |
|---|
| 10xMN-Medium | 3,68ml |
| Sterile water | 33,12ml |
| Glucose (20%) | 4ml |
| K-Glutamat (40%) | 200µl |
| Fe[III]-ammonium-citrate (2,2 mg/ml) | 200µl |
| Tryptophan (5 mg/ml) | 400µl |
| MgSO4 (1M) | 120µl |
| threonine (5 mg/ml)(in this case absent) | 400µl |
",
"tags" : "B.subtilis-PXyl"
}
"
