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Team:UCL/Labbook/Week10
From 2013.igem.org
Lab Weeks
Week 1 | Week 2 | Week 3 | Week 4 | Week 5 | Week 6 | Week 7 | Week 8 | Week 9 | Week 10 | Week 11 | Week 12 | Week 13 | Week 14 | Week 15 | Week 16
Week 10
Bacterial Lab
Monday 5th August - The new batch of ampicillin was tested via transformation of competent cells with pSecTag2A. Plates were spread and incubated 37C o/n.
Tuesday 6th August - Results from yesterday’s transformation:
| Vial | Ampicillin Plate | Plasmid Insertion | Colony Count |
|---|---|---|---|
| 1 (Main) | Yes | Yes | 100+ |
| 2 (Positive Control) | No | Yes | 100+ |
| 3 (Negative Control) | Yes | No | 15 |
This indicates that there is still an issue with the ampicillin, possibly a problem with the stock powder used. A final test with both old and new ampicillin was carried out and compared without plasmid insertion.
Wednesday 7th August -
| Vial | Ampicillin Plate | Plasmid Insertion | Colony Count |
|---|---|---|---|
| 1 Old Ampicillin | Yes | Yes | 100+ |
| 2 New Ampicillin v2 | No | Yes | 100+ |
| 3 Positive Control | Yes | No | 15 |
Results indicated that the ampicillin source may not have been fully functional, therefore a new source of amp powder was located and amp was remade.
Thursday 8th August - Preparation of 4x Amp plates and 4x no drug plates for storage in the fridge.
Friday 9th August - Meeting with Darren Nesbeth, requirements for upcoming weeks: create glycerol stocks of both pSecTag2A and pSB1C3, purify and extract pure DNA for pSecTag2A and pSB1C3.
Mammalian Lab
Tuesday 6th August - Checked HeLa cells. Cells are growing slow, left to grow for a few more days
Thursday 8th August - HeLa cells are about 30% confluent. Changed media.
