Team:Paris Bettencourt/Notebook/Trojan Horse/Wednesday 28th August.html

Trojan Horse

28^th August

as expected MGZ1 and MG1655 turn blue.

Result from assemblies:

Gibson sRNA LacZ in litmus-GFP: failure

Biobrick assembly sRNA LacZ in Litmus-GFP : success

Biobrick assembly sRNA LacZ in pUC18 : success

Miniprep of Litmus-GFP-sRNACm ,Litmus-GFP-sRNALacZ, pUC18-sRNACm , pUC18-sRNALacZ

Building phagemid weapon producing cells: Transformation of electrocompetent MGZ1, F+ with M13K07 + Litmus-GFP-sRNALacZ / Litmus-GFP-sRNACm

Transforming:

-1) negative control : 5,2

-2) IDT plasmid with sRNALacZ ( should form white colonies of MGZ1 on Xgal+IPTG):5,1

-3) Litmus-GFP-sRNALacZ1 ( should form white colonies of MGZ1 on Xgal+IPTG): 5,2

-4) Litmus-GFP-sRNALacZ3 ( should form white colonies of MGZ1 on Xgal+IPTG): 5,2

-5) Litmus-GFP-sRNACm1 (control XGal ) : 5,2

-6) Litmus-GFP-sRNACm1 + M13K07: 5,2

-7) Litmus-GFP-sRNACm2 + M13K07 : 5,2

-8) Litmus-GFP-sRNACm3 + M13K07: 5,2

-9) Litmus-GFP-sRNALacZ1 + M13K07 : 5,2

-10) Litmus-GFP-sRNALacZ3 + M13K07: 5,2

plate on AMP+IPTG+Xgal ( silencing of LacZ on MGZ1 strain) and KAN+AMP (phage factories cells)