Team:Paris Bettencourt/Notebook/Trojan Horse/Friday 26th July.html

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Trojan Horse

26th July



Infectiveness characterization experiments
  • Dilute 1/100 MGZ1, F+ O/N culture in 10mL LB => wait OD(600) = 0.7
  • Centrifuge M13K07 4000rpm, 10 minutes
  • Retrieve surnageant and filter it with 0.2 filter
  • Once OD 0.7 is reached, make 4 tubes
    1. 1mL MGZ1,F+ + 100ul LB
    2. 1mL MGZ1,F+ + 100ul surnageant diluted 1/100
    3. 1mL MGZ1,F+ + 100ul surnageant diluted 1/1000
    4. 1mL MGZ1,F+ + 100ul surnageant diluted 1/1000
  • Incube 37°C for 45 minutes
  • Serial dilute the tubes 10^-1, 10^-2, 10^-3, 10^-4, 10^-5, 10^-6
    • 4 times 6 tubes with 900uL LB, add 100ul of one previously made tube, mix and transfert 100ul to next tube.
  • Plate on LB (10^-6), Kan(10^-2), Amp(10^-6), Kan and Amp(10^-3)

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