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Up until now we succeded
• Producing Nanoparticles and loading them
• Clone a pH sensitive promoter
• Engineer a fusion protein between ice nucleation protein and streptavidin
But we didn't have enough time to characterize completely and assemble the parts to form the Taxi.Coli.
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What we would have done with more time:
Nanoparticles
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Cell surface display of Streptavidin
• Cloned a plasmid that encodes a fusion protein INP-Streptavidin-YFP and one INP-YFP-Streptavidin. Then we would have been able to use the exact same protocol we used to characterize the Biobrick BBa_K523013, to proof surface localization.
• Cloned a plasmid that encodes a fusion protein of INP with for example protein A to check if the
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Sensing/Effector
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Taxi.Coli
• Cloned one of our Biobricks in a backbone that contains another resistance than chloramphenicol to cotransfect bacteria with the Plasmids responsible for Sensing/Effector and the Plasmid used to connect the Nanoparticles.
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