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Team:Heidelberg/Tyrocidine week16 biobrickmod
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Contents |
RFC10 Standardization of Tyc Modules
Primer Design
Amplification of Tyc A
File:20130817.svg
TycA&B1 after PCR
| TycA | µl |
|---|---|
| Bacillus | 1 |
| AT01 | 2 |
| AT02 | 2 |
| Q5 2x Master mix | 10 |
| ddH20 | 5 |
| Cycles | temperature [°C] | Time [min:s] |
|---|---|---|
| 1 | 98 | 2:00 |
| 35 | 98 | 0:10 |
| 59 | 0:30 | |
| 72 | 1:30 | |
| 1 | 72 | 2:00 |
| 1 | 10 | inf |
Result There was a band at the right length but we were not able to cut.
Amplification of Tyc B1
A
File:20130817.svg
TycA&B1 after PCR
| TycB1 | µl |
|---|---|
| Bacillus | 1 |
| AT03 | 2 |
| AT04 | 2 |
| Q5 2x Master mix | 10 |
| ddH20 | 5 |
| Cycles | temperature [°C] | Time [min:s] |
|---|---|---|
| 1 | 98 | 2:00 |
| 35 | 98 | 0:10 |
| 56 | 0:30 | |
| 72 | 1:30 | |
| 1 | 72 | 2:00 |
| 1 | 10 | inf |
Result no band in gel, empty lane; optimization
