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Team:DTU-Denmark/Bioreactor
From 2013.igem.org
Bioreactor
Contents |
Description
The purpose of this experiment is to determine the long term characteristics of the growth of our Nir transformant anaerobically. Specifically, we measure nitrous oxide production over a period of 10 hours.
The experiment was performed on September 17th.
Methods
Protocol Bioreactor
For reasons that are not understood, the experiment became aerobic in each chamber just after 12:00, and the lab technician began sparging the reactors at a low rate to keep the reaction anaerobic.
Experiment timeline
- 10:00 Set up experiment; begin sparging with nitrogen to make the medium anaerobic
- 11:00 End sparging
- 14:00 Take first sample
- 22:00 Take last sample
Results
Nitrous oxide concentrations measured in the bioreactor at the beginning (t=14:00) and end (t=22:00) of the experiment.
| Time | [N2O] Replicate 1 | [N2O] Replicate 2 | [N2O] Replicate 3 | [[N2O] Distilled Water Control |
|---|---|---|---|---|
| 14:00 | 1.84 mg/L | 1.64 mg/L | 1.60 mg/L | N/A |
| 22:00 | 1.43 mg/L | 1.44 mg/L | 1.50 mg/L | 1.65 mg/L |
Discussion
The concentrations of nitrous oxide were slightly higher at the beginning of the experiment compared to the end, but none of the readings showed a large deviation from the control reading.
