Team:TU Darmstadt/protocols/DNA Quantification

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Bacterial cell culture

Materials

Equipment
Micropipettes with sterile tips
Nanodrop

Chemicals & consumables
DNA probe
Water

Procedure
1. Start the programme for the NanoDrop and click on "Nucleic Acids"
2. Pipette 1 µL of a water sample onto the lower measurement and click “OK” for initialising
3. Load your blank and click "Blank"
4. Load your DNA samples and click "Measure"

The system calculate automatically the 260/280 value and the 260/230 value.

References
http://openwetware.org/wiki/Bacterial_cell_culture

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