Team:TU Darmstadt/protocols/Glycerine stock

From 2013.igem.org

Revision as of 17:29, 4 October 2013 by Kai15 (Talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)





Glycerine stock

Short explanation
In order to have a permanent culture of cells glycerine stocks can be made.

Procedure
1. Add 200 µl of sterilized glycerine or DMSO to 800 µl cell culture and mix well
2. Freeze the stock at -20 °C