Team:TU Darmstadt/protocols/SDS-Page

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Labbook | Matierals | Protocols

Protocols


Equipment

  • - Two glass plates
  • - Gel caster
  • - Comb
  • - VWR Power Source 300V


Chemicals & consumables

  • - SDS
  • - Rotiphorese® (30%)
  • - Tris HCl
  • - Glycine
  • - TEMED
  • - APS
  • - Aqua dest.
  • - Isopropyle alcohol


Buffers

  • - Separation buffer (18.17 g Tris, 0.40 g SDS, 90 ml Aqua dest., pH = 8.8)
  • - Rotiphorese® (30%)
  • - Tris HCl
  • - Glycine
  • - TEMED
  • - APS
  • - Aqua dest.
  • - Isopropyle alcohol


Procedure

  1. prepare the separating gel and fill it into the chamber
  2. pour 1 mL isopropyle alcohol on the top of the gel to destroy air bubbles and prevent dehydration
  3. discard the isoprpyl alcohol and pour the prepared stacking gel
  4. stick in the comb
  5. store the gel in wet cloth (to prevent dehydration) at 4°C

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