Team:NTNU-Trondheim/Primers
From 2013.igem.org
![header](https://static.igem.org/mediawiki/2013/5/55/Logo-ntnu.png)
Primers
tat-signal sequence | |
---|---|
Primer | Sequence |
F_pl.b_tat | 5'- AGAGAAAGAGGAGAAATACTAGatggccaataacgatctctttcaggcatcacg-3' |
F_prefix_tat | 5'- GAATTCGCGGCCGCTTCTAGatggccaataacgatctctttcaggcatcacg-3' |
R_tat | 5'-cgcttgcgccgcagtcgcacgtcg-3' |
R_GFP_tat | 5'-CTTCTCCTTTACGCATcgcttgcgccgcagtcgcacgtcg -3' |
R_CFP_tat | 5'-GCCCTTGCTCACCATcgcttgcgccgcagtcgcacgtcg-3' |
SYFP | |
F_I_syfp | 5'-GGTGGATCCGGTGGATCAGGTGGATCCGGTGGTAGTatggttagcaagggcgaaga-3' |
R_syfp | 5'-GCCTTTCGTTTTATTTGATGCCTGGttattatttatacagctcatccatacccaggg-3' |
BFP | |
F_tat_BFP | 5'- CGACGTGCGACTGCGGCGCAAGCGatgagcgaactgatcaaagag-3' |
R_I_bfp | 5'-ACTACCACCGGATCCACCTGATCCACCGGATCCACCattcagtttatgacccagcttgc-3' |
GFP | |
F_tat_GFP | 5'-CGACGTGCGACTGCGGCGCAAGCGatgcgtaaaggagaagaact-3' |
R_I_gfp | 5'-ACTACCACCGGATCCACCTGATCCACCGGATCCACCtttgtatagttcatccatgcc-3' |
RFP | |
F_I_rfp | 5'- GGTGGATCCGGTGGATCAGGTGGATCCGGTGGTAGTatggcttcctccgaagacg-3' |
R_pl.b_rfp | 5'- GCCTTTCGTTTTATTTGATGCCTGGgcgatctacactagcactatcagcg-3' |
GFPmut3 | |
F_tat_GFPmut3 | 5'-CGACGTGCGACTGCGGCGCAAGCGagaaaaggagaagaacttttcactgg-3' |
R_pl.b_GFPmut3 | 5-'GCCTTTCGTTTTATTTGATGCCTGGttatttgtatagttcatccatgcc-3' |
CFP | |
F_tat_CFP | 5'-CGACGTGCGACTGCGGCGCAAGCGatggtgagcaagggcgaggagctgttc-3' |
R_l_CFP | 5'- ACTACCACCGGATCCACCTGATCCACCGGATCCACCcttgtacagctcgtccatgccgagag-3' |
YFP | |
F_l_YFP | 5'-GGTGGATCCGGTGGATCAGGTGGATCCGGTGGTAGTatggtgagcaagggcgaggagctgttc-3' |
R_pl.b_YFP | 5'- GCCTTTCGTTTTATTTGATGCCTGGttattacttgtacagctcgtccatgccg-3' |
Protein G | |
F_pl.b_PrG | 5'-AGAGAAAGAGGAGAAATACTAGatggaaaaagaaaaaaaggtaaaatac-3' |
F_tat_PrG | 5'-CGACGTGCGACTGCGGCGCAAGCGgttgactcaccaatcgaagatacccc-3' |
F_pl.b_pelB_PrB | 5'-AGAGAAAGAGGAGAAATACTAGATGAAATACCTGCTGCCGACCGCTGCTGCTGGTCTGCTGCTCCTCGCTGCCCAGCCGGCGATGGCC gttgactcaccaatcgaagatacccc-3' |
R_pl.b_PrG | 5'- GCCTTTCGTTTTATTTGATGCCTGGgatcggcatattgaaaaggc-3' |
R_pl.b_PrGstop | 5'-GCCTTTCGTTTTATTTGATGCCTGGttagtcttctttacgttttgaagcgac-3' |
Plasmid backbone | |
F_plasmid | 5'-ccaggcatcaaataaaacgaaaggc-3' |
F_RFP_pl.b | 5'-GTGCTAGTGTAGATCGCccaggcatcaaataaaacgaaaggc-3' |
F_YFP_pl.b | 5'-GAGCTGTACAAGTAATAAccaggcatcaaataaaacgaaaggc-3' |
R_pl.b | 5'- ctagtatttctcctctttctctagtagtgc-3' |
R_tat_pl.b | 5'- GAGATCGTTATTGTTCATctagtatttctcctctttctctagtagtgc-3' |
Sequencing Primers | |
F_sequence | 5'-tgccacctgacgtctaagaa-3' |
R_sequence | 5'-attaccgcctttgagtgagc-3' |