Team:BYU Provo/Notebook/SmallPhage/Fallexp/Period3/Dailylog

Small Phage

March-April

May-June

July-August

September-October

• Worked on putting together Jamboree presentation and poster.

• Tried to take EM to verify mutant size - did not work very well: phage titer is too low

10/4/13 - 10/6/13

JL, LP

• North America Regional Jamboree! For more information click here.

10/7/13

JL

• Started approximately 10 mL E coli B liquid culture overnight.

10/8/13

JL

• Started 10.8 Characterization of Mutant Phage by propagating the mutants.

10/9/13

JL, LP

• Purified the propagated mutant from 10.8 Characterization of Mutant Phage.

• Started approximately 20 mL of E coli B liquid culture overnight.

10/10/13

LP

• Performed spot tests to estimate the mutant phage titer in 10.8 Characterization of Mutant Phage.

• Started approximately 20 mL of E coli B liquid culture overnight.

JL

• Designed primers for cloning the major and minor capsid proteins into the iGEM registry: BI309 (Xbal) and BI310 (SpeI)

10/11/13

JL, LP

• Plated the mutant phage at -7 to verify their phenotypic stability. For specifics, please see 10.8 Characterization of Mutant Phage.

• Designed primers for more accurate sequencing: BI319 (160bp upstream from start codon) and BI320 (-160 downstream from minor capsid protein stop codon)

10/12/13

JL

• Started approximately 15mL of E coli B liquid culture overnight

10/13/13

JL

• Plated more phage from CsCl gradient to select for mutant. Specifics are recorded in 10.8 Characterization of Mutant Phage.

9/27/13

JL, LP

• Determined results for 9.13 Mutagen Concentration Test - Ninth Protocol.

• Finished updating the wiki.

• Started approximately 30mL of E. coli B overnight.