Team:BYU Provo/Notebook/LargePhage/Summerexp/Period10/Dailylog

Large Phage September - October Notebook: October 1 - October 15 Daily Log

9/16/13

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9/18/13

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9/23/13

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9/25/13

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10/9/13

Today we planned out a schedule that we will need to follow closely in order to have more data to present at the world jamboree at MIT. Our goals are to: Isolate a new large phage by running multiple new mutagenesis procedures Characterize mutations using PCR Clone the MCP and Capsid Vertex Protein into the registry (which requires point mutations to remove restriction sites)

10/10/13

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10/11/13

Today we designed primers for the mutagenesis we'll need to do to remove illegal restriction sites in the T4 MCP and capsid vertex proteins.

We also ran PCR on plaques from T4 WT and from liquid from sample 21-7 which was our small mutant that was supposed to be large. The PCR was run using these ratios:

To a eppendorf tube, add 120 ul ddH20 15 ul 10X TAQ Buffer 4.5 ul 10mM dNTP's 3 ul of forward primer 3 ul of reverse primer 1.5 uL Taq Polymerase

50 uL in each PCR tube

_ uL of template

10/14/13

Today we need to: Run a gel of our PCR products Start culturing our picked plaque (T4 WT to be used for mutagenesis)