Team:BYU Provo/Notebook/Phage Purification/Fallexp/Period3/Dailylog

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Phage Purification September - October Notebook: October 21- 27 Daily Log

Phage Purification

March-April

10/21/13

-Today we ran PCR on T4 wild type and mutant phage to determine the sequence differences in the capsid proteins.

AB DL AC

10/23/13

-Ran a gel on the PCR we did last time.

-Gel

100 mL TAE 1x

1 g regular agarose

2 drops ethidium bromide

Combine and melt TAE and agarose

Cool and add 2 drops ethidium bromide

Allow gel to form with 14 well mold (20 minutes in the fridge)

-Preparing machine

Fill machine with TAE buffer until it covers gel

2 microL DNA ladder in first well

2 microL stain and 5 microL sample, mix and put in well

We place the samples in the order: DNA ladder, 297/298 W M, 301/302 W M, 311/312 W M, 313/314 W M W=wildtype M=mutant

AB DL AC