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Team:Paris Bettencourt/Notebook/Phage Sensor/Thursday 24th October.html
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Detect
ASDFThursday 24th October
Digest phagemid E/P
Purify it
Ligation:
1. SPCR3 E/P + phagemid E/P
2. SPCR3 E/P + SPCR5 P/X+ phagemid E/X
2. SPCR3 E/P + SPCR2 P/X+ phagemid E/X
trafo Ligations
electrocmpetent cells parental+sSP009+pS002’
trafo:
1. parental+pS002
2. parental+pS002’
3. parental+pS006*
4. parental+pS002’+pS006*
5. sSP009 pS002’ + pS006*
trafo helper into NEB
liquid cultures: sSP009, pRECA strains Ariel, sSP008+pS002, sSP008+pS002’
