Team:XMU-China/Content parts

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Parts

Table 1. The Bio-bricks used in the study

Part	Backbone	Type	Location	Size (bp)		Description
Part	Backbone	Type	Location	part	backbone	Description
BBa_K546000	pSB1C3	Signaling	2013-P1-12D	1964	2070	Lux pL controlled LuxR with lux pR autoinducing LuxI (lva tag)- AHL.
BBa_I763020	pSB1C3	Intermediate RBS-GFP-TT	2013-P3-11H	914	2070	RBS-GFP (+LVA Tag)-Terminators
BBa_F2621	pSB1A2	Signaling	2013-P2-21F	1158	2079	3OC6HSL Receiver Device
BBa_K546001	pSB1C3	Device	2013-P1-12F	2135	2070	AIIA AHL Reporter and Quencher
BBa_J04450	pSB4K5	Reporter	2013-P5-5G	1069	3419	RFP Coding Device
BBa_J04450	pSB3T5	Reporter	2013-P5-7C	1069	3241	RFP Coding Device
BBa_J23022	BBa_J23006	Composite	2013-P5-3H	234	2356	[key3d][TT]

Favorite XMU-China 2013 iGEM Team Parts

W/N	Name	Type	Description	Designer	Length
W	BBa_K1036000	Composite	lux pL controlled luxR with lux pR controlled ndh (LVA-tag) coding for NADH dehydrogenase II	Shengquan Zeng Zhaopeng Cheng	2687
W	BBa_K1036001	Coding	the ndh gene coding for respiratory NADH dehydrogenase II	Shengquan Zeng Zhaopeng Cheng	1366
W	BBa_K1036003	Composite	lux pL controlled luxR with lux pR controlled gfp (LVA-tag)	Xin Guo Xi Xi	4052
W	BBa_K1036006	Composite	lux pL controlled luxR with lux pR controlled sfgfp (with LVA-tag)	Zhaopeng Cheng Xiyu Wu	4046

XMU-China 2013 iGEM Team Parts Sandbox

W/N	Name	Type	Description	Designer	Length
W	BBa_K1036002	Composite	gfp (with LVA-tag) under plux R control	Xin Guo Xi Xi	2866
W	BBa_K1036004	Reporter	sfgfp with LVA-tag	Zhaopeng Cheng Xiyu Wu	753
W	BBa_K1036005	Composite	sfgfp (with LVA-tag) under plux R control	Zhaopeng Cheng Xiyu Wu	2880

Agarose Gel Electrophoresis Confirmation for all Plasmids

pSB1C3-gfp-luxI

This plasmid consists of two parts of target genes: gfp and luxI, and both of them are regulated by quorum sensing promoter. High copy number. (Fig.7-1)

Fig.7-1 AGE confirmation of plasmid pSB1C3-gfp-luxI construction

pSB1C3-sfgfp-luxI

This plasmid consists of two parts of target genes: sfgfp and luxI, and both of them are regulated by quorum sensing promoter. sfGFP gene is included into this plasmid as a parallel experiment group of pSB1C3-gfp-luxI. High copy number. (Fig.7-2)

Fig.7-2 AGE confirmation of plasmid pSB1C3-sfgfp-luxI construction

p3H-GFP-luxI

This plasmid consists of two parts of target genes: gfp and luxI, and both of them are regulated by quorum sensing promoter. Middle copy number. (Fig.7-3)

Fig.7-3 AGE of p3H-gfp construction

p3H-sfGFP-luxI

This plasmid consists of two parts of target genes: sfgfp and luxI, and both of them are regulated by quorum sensing promoter. sfGFP gene is included into this plasmid as a parallel experiment group of pSB1C3-gfp-luxI. Middle copy number. (Fig.7-4)

Fig.7-4 AGE of p3H-sfgfp construction

pSB3T5-aiiA

This plasmid has one target gene aiiA under the regulation of quorum sensing promoter. Middle copy number. (Fig. 7-5)

Fig.7-5 AGE confirmation of plasmid pSB3T5-aiiA construction

pSB4K5-ndh

This plasmid has one target gene aiiA under the regulation of quorum sensing promoter. Low copy number. (Fig. 7-6)

Fig.7-6 AGE confirmation of plasmid pSB4K5-ndh construction