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The transformation from the day before worked. There were no colonies is the insert and the competent cells control as expected. There were colonies in the backbones control and in the gibson transformation plates.
1) Colony PCR verification
- 0.8% Gel electrophoresis to verify the colony PCR of the day before.
- we obtained amplifications for colonies 1,3,5,6,7 of IYS and 1,4,6 of ISY.

2) Microscopy
- samples from IYS colonies 1,3,5,6 and ISY colonies 1,3,4,6 were prepared acording to our protocols.
- phycoerythrin conjugated anti-GFP antibody , FITC conjugated anti-Streptavidin antibody and Fitc conjugated biotin were used.