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AutoAnnotator by Team TU-Munich 2013

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Sample Output

Automatically determined parameters using the [http://www.mediawiki.org/wiki/Table BioBrick-AutoAnnotator]
BioBrick: BBa_K1159000 Used open reading frame from position ?x? to ?y?.
A (Ala) 1 (5.00%) R (Arg) 1 (5.00%) N (Asn) 1 (5.00%) D (Asp) 2 (10.00%) C (Cys) 1 (5.00%)
Q (Gln) 1 (5.00%) E (Glu) 2 (10.00%) G (Gly) 1 (5.00%) H (His) 1 (5.00%) I (Ile) 0 (0.00%)
L (Leu) 0 (0.00%) K (Lys): 1 (5.00%) M (Met) 1 (5.00%) F (Phe) 2 (10.00%) P (Pro) 1 (5.00%)
S (Ser) 1 (5.00%) T (Thr) 0 (0.00%) W (Trp) 1 (5.00%) Y (Tyr) 1 (5.00%) V (Val) 1 (5.00%)
Amino acid counting: Total number of amino acids (aa): 20 Number of positively charged aa (Arg + Lys): 2 Number of negatively charged aa (Asp + Glu): 4
Biochemical parameters: Molecular weight [Da]: 2459.69 Theoretical pI: 4.75 Extinction coefficient: 7052.5 (all Cys as cystine), 6990 (no Cys as cystine)
Estimated half-life: Mammals: 4.4 hour Yeast: >20 hour E. coli: >10 hour
Codon usage: Mammals: ?good? Yeast: ?Ok? E. coli: ?bad ?
RFC standard: This is a RFC 25 BioBrick, thus ATGGCCGGC and ACCGGT were added to the 5' and 3' ends.
The BioBrick-AutoAnnotator was created by TU-Munich 2013 iGEM team. For information please read the description.