Summary

In this summer, we construct a new alkane sensing system AlkR-PalkM, all the achievements are based on this system.

1.Based on the system of AlkR-PalkM , we use this to build an Alk-Sensor based on the system of AlkR-PalkM which can detect the alkane which is produced by engineered E. coli.

2.We characterized the Alk-Sensor of different aspects.

3.Based on Alk-Sensor, we constructed Alk-Selector for direct evolution

4.We used Alk-Selector to optimize the function of NPDC.

Future work

All parts are also not perfect, needed to be optimized.

1. 1.It may be necessary to mutate PalkM to reduce the leakage of the system.

1. 2.Different reporter needed to be tried to get different relationship between input and output.

1. 3.Mutate AlkR may promote the interaction between AlkR and Alkane, and improve the function of AlkR-PalkM system.

1. 4.Addition of gene related to biosufactant biosynthesis may increasing the sensing range of the alkane

1. 5.For biofuel synthetic, Alk-Selector provide the more opportunities to engineered the strain. Many method related to build a library can be used in this field with the help of Alk-Selector.