BioBrick

Favorite Tianjin 2013 iGEM Team Parts

Name	Type	Description	Designer	Length
Bba_K1020005	Composite	Alkane Marker--Device for detection of various alkanes	Boxuan Zeng Yue Liu	2146bp
Bba_K1020012	Composite	Alkane Selector for intracellular alkane production	Boxuan Zeng Yue Liu	2662bp

Name	Type	Description	Designer	Length
Bba_K1020001	Reporter	PalkM promoter with reporter gene	Boxuan Zeng Yue Liu	1013bp
Bba_K1020002	Coding	alkane regulatory protein ALKR	Boxuan Zeng	927bp
Bba_K1020003	Reporter	AlkR -PSB1C3-Low constitutive	Yue Liu	1125bp
Bba_K1020004	Composite	Biosensor for alkane detection	Yue Liu Su Wang	2009bp
Bba_K1020006	Coding	YbhGFSR	Boxuan Zeng Yang Wu	4971bp
Bba_K1020007	Composite	AlkR -PSB1C3-High constitutive	Yue Liu	1125bp
Bba_K1020008	Composite	alkane producing module	Boxuan Zeng Yue Liu	1809bp
Bba_K1020009	Composite	YbhGFSR operon	Boxuan Zeng	5034bp
Bba_K1020010	Coding	TetA gene under the control of PalkM	Boxuan Zeng Shoujie Sun	1529bp
Bba_K1020011	Composite	High expressed Alkane Selector for intracellular alkane production	Boxuan Zeng Qingyi Deng	2662bp

Results for Agarose Gel Electrophoresis:

Figure 1. AlkR-1 ~600bp cut from AlkR-1-pEASY-BLUNT

Figure 2. AlkR-2 ~600bp cut from AlkR-2-pEASY-BLUNT

Figure 3. J23100/J23103-AlkR-tt-RFP ~2000bp cut from J23100/J23103-AlkR-tt-RFP-pEASY-BLUNT

Figure 4. J23100/J23103-AlkR-tt-PalkM-RFP ~2000bp cut from J23100/J23103-AlkR-tt-PalkM-RFP-pSB1C3

Figure 5. J23100/J23103-AlkR-tt-PalkM-tetA ~2400bp cut from J23100/J23103-AlkR-tt-PalkM-tetA-pSB1C3

Figure 6. J23100/J23114-NPDC-AAR ~2000bp cut from J23100/J23114-NPDC-AAR-pSB3K3

Figure 7. The visible color change of the strain cultured in 3ML M9 medium with different induced for 12 hours.

Figure 8. Strain one contains alkane producer part(left),another contains none-producer part (right) .