BioBrick

Favorite Tianjin 2013 iGEM Team Parts

Name	Type	Description	Designer	Length
Bba_K1020005	Composite	Alkane Marker--Device for detection of various alkanes	Boxuan Zeng Yue Liu	2146bp
Bba_K1020012	Composite	Alkane Selector for intracellular alkane production	Boxuan Zeng Yue Liu	2662bp

Name	Type	Description	Designer	Length
Bba_K1020001	Reporter	PalkM promoter with reporter gene	Boxuan Zeng Yue Liu	1013bp
Bba_K1020002	Coding	alkane regulatory protein ALKR	Boxuan Zeng	927bp
Bba_K1020003	Reporter	AlkR -PSB1C3-Low constitutive	Yue Liu	1125bp
Bba_K1020004	Composite	Biosensor for alkane detection	Yue Liu Su Wang	2009bp
Bba_K1020006	Coding	YbhGFSR	Boxuan Zeng Yang Wu	4971bp
Bba_K1020007	Composite	AlkR -PSB1C3-High constitutive	Yue Liu	1125bp
Bba_K1020008	Composite	alkane producing module	Boxuan Zeng Yue Liu	1809bp
Bba_K1020009	Composite	YbhGFSR operon	Boxuan Zeng	5034bp
Bba_K1020010	Coding	TetA gene under the control of PalkM	Boxuan Zeng Shoujie Sun	1529bp
Bba_K1020011	Composite	High expressed Alkane Selector for intracellular alkane production	Boxuan Zeng Qingyi Deng	2662bp

Results for Agarose Gel Electrophoresis:

Figure 1. AlkR-1 ~600bp cut from AlkR-1-pEASY-BLUNT

Figure 2. AlkR-2 ~600bp cut from AlkR-2-pEASY-BLUNT

Figure 3. J23100/J23103-AlkR-tt-RFP ~2000bp cut from J23100/J23103-AlkR-tt-RFP-pEASY-BLUNT

Figure 4. J23100/J23103-AlkR-tt-PalkM-RFP ~2000bp cut from J23100/J23103-AlkR-tt-PalkM-RFP-pSB1C3

Figure 5. J23100/J23103-AlkR-tt-PalkM-tetA ~2400bp cut from J23100/J23103-AlkR-tt-PalkM-tetA-pSB1C3

Figure 6. J23100/J23114-NPDC-AAR ~2000bp cut from J23100/J23114-NPDC-AAR-pSB3K3

Characterization for our construction:

Figure 7. The visible color change of the strain cultured in 3ML M9 medium with different induced for 12 hours.

Figure 8. Strain one contains alkane producer part(left),another contains none-producer part (right) .