Team:BostonU/Protocols

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Protocols

This section contains the protocols that we used during the summer and that are mentioned in our Notebook.

We hope they help anyone interested in our experiments to successfully repeat them.


DNA Quantification Gel Extraction Miniprep
PCR Cleanup Ligation Media Prep
MoClo Level 0 Reaction MoClo Level 1 and 2 Reactions Phusion PCR
Restriction Digest Restriction Digest 10 µL mapping Transformation