Characterization Notebook

Goal: Develop a well-characterized library of parts for the MoClo Assembly Method

Subgoals:

Assemble level 0 parts needed

Constitutive Promoter Characterization Project:

J23100_AB, J23113_AB, J23115_AB, J23115_EB, J23116_AB, J23116_EB, J23117_AB, J23118_AB, J23119_AB
BCD1_BC, BCD2_BC, BCD8_BC, BCD12_BC, BCD13_BC, BCD16_BC: completed 6/27/13

Repressible Promoter Characterization Project:

C0012 (LacI), C0062_CD (LuxR), C0071_CD (rhlR_CD), pLacI (R0010_FB)

Assemble level 1 circuits for characterization of Constitutive Promoter Library

160 circuits that have different combinations of J231XX promoters and RBS’s

1 set with all promoters + BCD2
1 set with all RBS’s+J23100

Assemble level 1 circuits for characterization of Repressible Promoter
Run flow cytometry on the promoter characterization circuits
Assemble level 2 inverter as an example of the library’s use
Switch the backbone in some existing level 1 parts to study how different antibiotics affect the flow cytometry data: completed:6/21/13

May
Week of May 12, 2013

Learned about synthetic biology, MoClo, and developed plans for our summer projects, specifically histidine kinase and quorum sensing.

Learned about Clotho software suite and learned how to use Eugene, RavenCAD, and Pigeon from other members of the CIDAR lab.

Familiarized ourselves with protocols and lab etiquette

Stocked up on lab supplies (plates, broth, plasmids)

Retrieved from "http://2013.igem.org/Team:BostonU/NotebookML"