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From 2013.igem.org

Enter a BioBrick number below, either starting with "BBa_" or just the alphanumeric code!
Alternatively (e.g. for new BioBricks, which aren't in the Registry yet) you can enter the nucleotide sequence directly.

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BioBrick: BBa_

Automatically determined parameters using the BioBrick-AutoAnnotator version 1.0

RFC standard:

RFC 10 BioBrick

ORF from 3 to 23 (excluding stop-codon)

Nucleotide sequence: (underlined part encodes the protein, italic parts were added)  ATATGGGGTTA ... GTATACTCCTAGC

Amino acid sequence:   1 MGLLVYS

Amino acid composition:

A (Ala)

0 (0.0%)

C (Cys)

0 (0.0%)

H (His)

0 (0.0%)

M (Met)

1 (14.3%)

T (Thr)

0 (0.0%)

R (Arg)

0 (0.0%)

Q (Gln)

0 (0.0%)

I (Ile)

0 (0.0%)

F (Phe)

0 (0.0%)

W (Trp)

0 (0.0%)

N (Asn)

0 (0.0%)

E (Glu)

0 (0.0%)

L (Leu)

2 (28.6%)

P (Pro)

0 (0.0%)

Y (Tyr)

1 (14.3%)

D (Asp)

0 (0.0%)

G (Gly)

1 (14.3%)

K (Lys)

0 (0.0%)

S (Ser)

1 (14.3%)

V (Val)

1 (14.3%)

Amino acid counting:

Total number:

7

Positively charged (Arg + Lys):

0 (0.0%)

Negatively charged (Asp + Glu):

0 (0.0%)

Biochemical parameters:

Molecular mass [Da]:

782.0

Theoretical pI:

5.27

Extinction coefficient at 280 nm [M-1 cm-1]:

1490 / 1490 (all Cys red./ox.)

Estimated half-life [h]:

E. coli:

>10

S. cervisiae:

>20

Mammals:

30

Codon usage (CAI):

E. coli:

acceptable (0.55)

S. cervisiae:

good (0.63)

Mammals:

acceptable (0.42)

The BioBrick-AutoAnnotator was created by TU-Munich 2013 iGEM team. For more information please read the description.

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