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Team:Groningen/Labwork/25 June 2013
From 2013.igem.org
Mirjam, Chaline and Inne
A couple of media are prepared:-LB medium with agar 1 liter
-LB medium 0.5 liter divided into 60/80 ml portions
-70% ethanol
-1x TBE buffer 2 liter
Both LB media are autoclaved together with tooth picks, 1.5 and 2 ml eppendorf tubes. After autoclaving, the LB medium with agar is placed in the 55°C stove.
Mirjam and Claudio
B. subtilis strain 168 is inoculated in LB medium O/N at 37°C and every buffer necessary for the extraction of genomic DNA is arranged.
