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Team:Groningen/Labwork/26 June 2013
From 2013.igem.org
Mirjam
Did a genomic DNA extraction of B. subtilis strain 168. Two tubes are obtained from this with a concentration of 66ng/µl and 67ng/µl.The primers for our main subject, silk secretion, arrived. The primers are diluted to a concentration of 10mM.
To run a PCR, dNTPs are necessary in a concentration of 10mM, so this dilution is also made.
A PCR reaction mix is made for the four different signal sequences.
| signal sequence | Tm-5 | size |
|---|---|---|
| FliZ | 45°C | 130 |
| MotB | 50°C | 100-150 |
| EstA | 50°C | 100-150 |
| LytB | 50°C | 100-150 |
The PCR product is loaded on gel to examine if the PCR reaction succeeded.
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