29/07/13

From 2013.igem.org

Revision as of 13:38, 29 July 2013 by Queziatoe (Talk | contribs)
  1. Ran the gel from the 26/07/2013 again for 45min
  2. Digestion of plasmid backbone (pSB1C3)
  • Three different reactions
  • Master Mix (changes were made from the protocol)
    • 5ul NEB Buffer 2
    • 0.5ul of EcoRI
    • 0.5ul of PstI
    • 19ul of dH2O
  • For each reaction add:
    • 4ul of linearized backbone
    • 4ul of enzyme master mix
  • digest at 37C for 30min
  • heat kill at 80C for 20min
Retrieved from "http://2013.igem.org/29/07/13"