Team:UTK-Knoxville/5 June 2013
From 2013.igem.org
June 5th 2013
Today we isolated OmpR again. We used the POmpR_F and POmpR_R primers. Testing 60 C and 64 C. This time we used the Protocol for Q5® High-Fidelity 2X Master Mix on NEB’s website, as opposed to the slightly modified phusion protocol we have been using.
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