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From 2013.igem.org

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Parameters for BioBrick BBa_K801090 automatically created by the BioBrick-AutoAnnotator version 1.0
Nucleotide sequence in RFC 25, so ATGGCCGGC and ACCGGT were added (in italics) to the 5' and 3' ends: (underlined part encodes the protein)  ATGGCCGGCTACACAATG ... ATGCTCGCTACCGGT  ORF from -8 to 2160 (excluding stop-codon)
Amino acid sequence:   1 MAGYTMAPSLDSISHSFANGVASAKQAVNGASTNLAVAGSHLPTTQVTQVDIVEKMLAAPTDSTLELDGYSLNLGDVVSAARKGRPVRVKDSDEIRSKID 101 KSVEFLRSQLSMSVYGVTTGFGGSADTRTEDAISLQKALLEHQLCGVLPSSFDSFRLGRGLENSLPLEVVRGAMTIRVNSLTRGHSAVRLVVLEALTNFL 201 NHGITPIVPLRGTISASGDLSPLSYIAAAISGHPDSKVHVVHEGKEKILYAREAMALFNLEPVVLGPKEGLGLVNGTAVSASMATLALHDAHMLSLLSQS 301 LTAMTVEAMVGHAGSFHPFLHDVTRPHPTQIEVAGNIRKLLEGSRFAVHHEEEVKVKDDEGILRQDRYPLRTSPQWLGPLVSDLIHAHAVLTIEAGQSTT 401 DNPLIDVENKTSHHGGNFQAAAVANTMEKTRLGLAQIGKLNFTQLTEMLNAGMNRGLPSCLAAEDPSLSYHCKGLDIAAAAYTSELGHLANPVTTHVQPA 501 EMANQAVNSLALISARRTTESNDVLSLLLATHLYCVLQAIDLRAIEFEFKKQFGPAIVSLIDQHFGSAMTGSNLRDELVEKVNKTLAKRLEQTNSYDLVP 601 RWHDAFSFAAGTVVEVLSSTSLSLAAVNAWKVAAAESAISLTRQVRETFWSAASTSSPALSYLSPRTQILYAFVREELGVKARRGDVFLGKQEVTIGSNV 701 SKIYEAIKSGRINNVLLKMLATG*
Amino acid composition: A (Ala) 80 (11.1%) R (Arg) 35 (4.8%) N (Asn) 29 (4.0%) D (Asp) 30 (4.1%) C (Cys) 4 (0.6%) Q (Gln) 23 (3.2%) E (Glu) 41 (5.7%) G (Gly) 50 (6.9%) H (His) 26 (3.6%) I (Ile) 31 (4.3%) L (Leu) 87 (12.0%) K (Lys) 29 (4.0%) M (Met) 16 (2.2%) F (Phe) 21 (2.9%) P (Pro) 27 (3.7%) S (Ser) 69 (9.5%) T (Thr) 48 (6.6%) W (Trp) 4 (0.6%) Y (Tyr) 13 (1.8%) V (Val) 60 (8.3%)
Amino acid counting:   Total number: 723 Positively charged (Arg+Lys): 64 (8.9%) Negatively charged (Asp+Glu): 71 (9.8%)	Biochemical parameters: Molecular mass [Da]: 77561.4 Theoretical pI: 6.44 Extinction coefficient at 280 nm [M-1 cm-1]: 41370 / 41620 (all Cys red/ox)
Organism specific parameters Organism E. coli B. subtilis S. cervisiae A. thaliana Mammals Estimated half-life [h] >10 >20 30 Codon usage (CAI) good (0.63) B. subtilis acceptable (0.46) A. thaliana good (0.69)
Organism specific parameters Organism: E. coli B. subtilis S. cervisiae A. thaliana Mammals Estimated half-life [h] >10 >20 30 Codon usage (CAI) good (0.63) B. subtilis acceptable (0.46) A. thaliana good (0.69)
Sequence features:
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