06/08/13

From 2013.igem.org

Running of an agarose gel

• Running the gel for digests of samples 5.1, 10.1, 10.2 and 10.3 from the previous day.
• expectation-7kb band, confirming the presence of the limonene biobrick (5kb) and chloramphenicol backbone (2kb)

• The gel shows the presence of 7kb bands for both digests of samples 10.1, 10.2 and the 5.1 sample, which was expected.
• No bands appeared for samples 5.1.
• For samples 10.3 there is a 2kb band, indicating that with the ligation, the chloroamphenicol circulized.

Making chloroamphenicol agar plates

• Making additional plates
  • 400ml of agar
    • Melt in microwave
    • 3mins on low -> shake
    • Repeat
    • 2mins on low -> shake
    • Repeat
  • Add 800ul of chloroamphenicol at a concentration of 25 ul/ml
  • Pour out onto 20 petri dishes
  • Leave to set on bench

Plating out samples

• Plating out samples 10.1, 10.2, 10.3 and 5.1 onto chloroamphenicol plates.
• Grow overnight at 37C