Team:BostonU/Protocols

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iGEM 2012 main page

Protocols

This section contains the protocols that we used during the summer and that are mentioned in our Notebook.

We hope they help anyone interested in our experiments to successfully repeat them.

DNA Quantification
Gel Extraction
Miniprep
PCR Cleanup
Ligation
Media Prep
MoClo Level 0 Reaction
MoClo Level 1 and 2 Reactions
Phusion PCR
Restriction Digest
Restriction Digest 10 µL mapping
Transformation

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