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• iGEM 2013

Large Phage May - June Notebook
Overview March-April May-June July-August September-October	July 1 - July 15 Text Formating test Daily log Experiment Listing Progress Report
	July 16 - July 31 Text Daily log Experiment Listing Progress Report
	August 1- August 15 Add description! Daily log Experiment Listing Progress Report
	Date Add description! Daily log Experiment Listing Progress Report

Contents 1 July 1.1 7/1/13 1.2 7/3/13 1.3 7/5/13 1.4 7/8/13 1.5 7/10/13 1.6 7/12/13 1.7 7/15/13 1.8 7/17/13 1.9 7/19/13 1.10 7/22/13 1.11 7/24/13 1.12 7/26/13 1.13 7/29/13 1.14 7/31/13 2 August 2.1 8/2/13 2.2 8/5/13 2.3 8/7/13 2.4 8/9/13 2.5 8/12/13 2.6 8/14/13 2.7 8/16/13 2.8 8/19/13 2.9 8/21/13 2.10 8/23/13 2.11 8/26/13 2.12 8/28/13 2.13 8/30/13 3 September 3.1 9/2/13 3.2 9/4/13 3.3 9/6/13 3.4 9/9/13 3.5 9/11/13 3.6 9/13/13 3.7 9/16/13 3.8 9/18/13 3.9 9/20/13 3.10 9/23/13 3.11 9/25/13 3.12 9/27/13 3.13 9/30/13

July

7/1/13

7/3/13

7/5/13

7/8/13

Today we need to address some problems we ran into and make some plans for the future.

1. Plaque sizes aren't consistent, even though we are using the same agarose each time.
2. We haven't been able to observe plaque phenotypes staying the same through multiple generations
3. We have tried multiple liquid cultures and rounds of mutagenesis but we haven't been able to observe clearage in a flask yet.

Good things to model--
- Change in plaque size vs. agarose concentration
- Mutant vs wild type phage under UV - graph survival rates

We looked at a new procedure from the article "Genetic Control of Capsid Length in Bacteriophage T4" and found a much better and easier purification technique. We also read about centrifuge rotors here - http://bricker.tcnj.edu/tech/BIOL311centrifugation.html

7/10/13

7/12/13

7/15/13

7/17/13

7/19/13

7/22/13

7/24/13

7/26/13

7/29/13

7/31/13

August

8/2/13

8/5/13

8/7/13

8/9/13

8/12/13

8/14/13

8/16/13

8/19/13

8/21/13

8/23/13

8/26/13

8/28/13

8/30/13

September

9/2/13

9/4/13

9/6/13

9/9/13

9/11/13

9/13/13

9/16/13

9/18/13

9/20/13

9/23/13

9/25/13

9/27/13

9/30/13