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• iGEM 2013

Contents 1 July 2 August 2.1 8/2/13 2.2 8/5/13 2.3 8/7/13 2.4 8/9/13 2.5 8/12/13 2.6 8/14/13 2.7 8/16/13 2.8 8/19/13 2.9 8/21/13 2.10 8/23/13 2.11 8/26/13 2.12 8/28/13 2.13 8/30/13 3 September 3.1 9/2/13 3.2 9/4/13 3.3 9/6/13 3.4 9/9/13 3.5 9/11/13 3.6 9/13/13 3.7 9/16/13 3.8 9/18/13 3.9 9/20/13 3.10 9/23/13 3.11 9/25/13 3.12 9/27/13 3.13 9/30/13

July

August

8/2/13

Today we set up an experiment which we will do on 8/7/13. We re-picked the plaques from the plate of mutagenized phage. The plate was full of variations in plaque sizes. We too the biggest plaques and put them in a mL of LB broth in an eppendorf. We also started to take a closer look at our EMs from Wednesday. We are slowly learning how to use the program imageJ to our best use.

8/5/13

Today we spent class working with ImageJ to measure the phage from the various categories in the cesium chloride gradient.

8/7/13

Today we picked from small plaques and diluted them in 1 mL of LB. We didn't have grown bacteria so we just put the tubes in the fridge.

We also discussed the need to separate the phage from the CsCl gradient into fractions so we don't risk contaminating the large phage fraction with normal phage from the large, central band.

8/9/13

Results from 8/7/13 plaque test:

8/12/13

8/14/13

8/16/13

8/19/13

8/21/13

8/23/13

8/26/13

8/28/13

8/30/13

September

9/2/13

9/4/13

9/6/13

9/9/13

9/11/13

9/13/13

9/16/13

9/18/13

9/20/13

9/23/13

9/25/13

9/27/13

9/30/13