"
12/07/13
From 2013.igem.org
Revision as of 10:12, 14 August 2013 by AliceHaworth (Talk | contribs)
| Home | Team | Official Team Profile | Project | Parts Submitted to the Registry | Modeling | Notebook | Safety | Attributions |
|---|
Transformation of RFP control plasmid
- Keep competent cells on ice
- Add 50ul of cells to 4 tubes
- Add 1ul of resuspended DNA to 2 tubes
- Add 1ul RFP to the other 2 tubes
- Incubate for 30mins
- Heat shock at 42 degrees for 60secs
- Incubate on ice for 5mins
- Incubate at 37degrees for 2hrs
