Team:BYU Provo/Notebook/SmallPhage/Springexp

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Small Phage Spring Notebook



Overview

Winter

Spring

Summer

Fall

April 29 - May 12


This is the start of Spring Term at BYU! The weather is finally getting better: no more snowing. And we are ready to do some serious lab work! While we are waiting for our mutgen, 5-bromodeoxyuridine, to arrive, we decided to start this term with establishing the basic procedures for our direct evolution method. Specifically, this involves making sure that we can (1) grow and amplify T7 phage in liquid culture, (2) select for smaller phage using concentrated top agar, and (3) amplify phage from a single plaque. During these two weeks, we also designed primers for cloning and sequencing T7 capsid proteins as well as mapped out detailed procedures for both cloning T7 capsid proteins and applying mutagen.


Daily log

Experiment Listing

Progress Report

5.3 T7 phage selection method test
5.3 Phage Amplification/Purification

Spring1.JPG


May 13 - May 26
Add goals
link to Daily log
Experiments
Progress Report
Spring2.JPG


May 27 - June 9
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link to daily log
Experiments
Progress Report
Spring3.JPG


June 10 - June 23
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link to daily log
Experiments
Progress Report
Spring4.JPG