Team:BYU Provo/Notebook/SmallPhage/Springexp/Period1/Explist

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Small Phage Spring Notebook: April 29 - May 12 Daily Log



Overview

Winter

Spring

Summer

Fall

5.3 T7 phage selection method test

In this experiment, we aimed to test whether or not we can select for smaller phages using concentrated top agar.

Detailed procedure: 5.3 T7 phage selection method test


5.3 T7 phage amplification/purification

In this experiment, we aimed to establish a basic protocol for amplifying and purifying phage.

Detailed procedure: 5.3 T7 phage amplification/purification


5/3/13

- Performed agar test, focusing primarily on ×8

5.3 T7 phage selection method test

- Processed phage amplification

5.3 T7 phage amplification/purification


5/4/13

- Performed dilution series using stock 5.3 (-1 through -11)

- Started two 5mL overnights of BL21


5/5/13

- Spot test using stock 5.3 and its dilution series (from 5.4)

|5.3 T7 phage amplification/purification

- Started liquid culture for purification team (at around noon)

1mL of BL21 overnight + 4mL of LB + 100μL -2 phage (received stock)

- Started 5.5 amplification from a plaque test

5.5 Amplification from a plaque test


5/6/13

- Continued 5.3 T7 phage amplification/purification

- Performed liquid culture phage concentration test

5.6 T7+ Liquid Culture Phage Concentration Test


5/7/13

- Started two 5mL of E coli BL21 overnight

- Designed procedure for applying mutagen and selecting for T7


5/8/13

- Went over procedure for applying mutagen and PCR with Dr. Grose

- Performed spot tests under 5.6 T7+ Liquid Culture Phage Concentration Test

- Started two 5mL BL21 overnights

- Learned how to create LB plates


5/9/13

- Practiced with ×6 and ×8 top agar

5.9 T7 selection method test #2

- Started 5.9 T7+ Liquid Culture Phage Concentration Test #2

- Discussed plans and schedule for next week.