Team:Purdue/Notebook/Laboratory Notebook

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Laboratory Notebook not your high school lab notebook

If you want to follow the play by play action, now is your opportunity.

Contents

Wednesday 5/15/13

  • Amanda met with iGEM faculty advisor Prof. Clase about advisors, HHMI student (Austin) and wetlab involvement.
  • Amanda met with Prof. Tao about project modeling.


Sunday 5/19/13

  • James, Peter, Brenda Gonzalez and Arizona Fox went to Greenfield HS for Girl Scouts synthetic biology workshop.
  • Chris and Amanda met to talk about week and the summer.

Monday 5/20/13

  • Charlotte and Nidhi completed required lab training.
  • Chris and Charlotte met with the business office and filled out the required payroll forms.

Tuesday 5/21/13

  • Chris created all of the social media pages for the team, including Facebook, Twitter, Google+, Gmail, and the blog.
  • Everyone did general research about the different types of post-translational modifications.

Wednesday 5/22/13

  • Team Meeting (Biomakers assemble!!)
    • Post-Translational Modifications
    • Social media
    • iGEM wiki (Alec)
    • iGEm website
  • James and Xiuyuan met with Prof. Tao to discuss project modeling task outline.

Thursday 5/23/13

  • Meeting with Prof. Rickus
    • Taguchi statistical methods for design optimization
    • Changed project from PTMs to quality control and Taguchi methods
  • Austin arrived on campus... Good times...

Friday 5/24/13

  • Team Meeting
    • PTMs and biobricks
    • Robustness and quality control
    • Different assembly methods
    • Ways to measure gene expression
    • Create plan for next week
  • Started wiki design

Monday 5/27/13

  • Memorial Day

Tuesday 5/28/13

  • Team Meeting
    • Gave everyone a variable to research for quality control
    • Talked about public relations
  • Team researched generally for quality control project

Wednesday 5/29/13

  • Amanda met with Peter to discuss the project budget
  • Team researched into past iGEM project relating to quality control

Thursday 5/30/13

  • Exec meeting with Amanda, Chris and Jim to discuss project feasibility
  • Team meeting to discuss background research

Friday 5/31/13

  • Meeting with whole team, Janie, and Dr. Tao about project ideas
  • Created a 3 week plan


Monday 6/3/13

  • Meeting with Dr. Rickus, Austin and Amanda about Austin’s project for HHMI

* Discussed details of Taguchi method applied to genetic circuitry

Tuesday 6/4/13

  • Team meeting to further discuss yeast polarization project idea

Wednesday 6/5/13

  • Meeting with Dr. Rickus, Dr. Clase, Soo and Janie to discuss multiple project ideas, which are feasible and what direction to work in

Thursday 6/6/13

  • Meeting with Dr. Mosier to discuss the use of yeast and lab space
  • Meeting with Jenn to catch her up with project details

Friday 6/7/13

  • Meeting with Amanda and Tarun to discuss project ideas

Monday 6/10/13

Tuesday 6/11/13

  • Made BL21 competent E. coli cells

Wednesday 6/12/13

  • Meeting with Dr. Kishmairer about yeast polarization project

Thursday 6/13/13

Friday 6/14/13

  • Meeting with Larisa to discuss working with yeast cells in Bindley Bioscience Center

Monday 6/17/13

Tuesday 6/18/13

  • Meeting with Amanda and Austin to discuss progress on HHMI Robustness project

Wednesday 6/19/13

  • Lunch meeting with whole team
  • Met with Dr. Hazbun about yeast polarization

Thursday 6/20/13

  • Brainstormed fundraising ideas
  • Meeting with Dr. Briggs to discuss the use of yeast in lab and polarization project

Friday 6/21/13

  • Meeting with Dr. Clase to discuss presentation with teachers
  • Team meeting

Monday 6/24/13

  • Created a project outline and plan for the Taguchi Robustness project

Tuesday 6/25/13

Wednesday 6/26/13

  • Human Practices meeting with Ryan, Swetha, Betsy and Amanda

Thursday 6/27/13

  • Time line for Taguchi project with Mike

Friday 6/28/13

  • Team meeting with Mike to discuss project deadlines for the protein overexpression (BCD)

Monday 7/1/13

  • Meeting with Mike to discuss project deadlines for standardization of characterization

Tuesday 7/2/13

  • Meeting with Jill about using the Flow Cytometer in Bindley
  • HP meeting with Ryan, Swetha, Betsy and Amanda

Wednesday 7/3/13

  • Team meeting about project

Thursday 7/4/13

Friday 7/5/13

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Monday 7/8/13

  • Exec meeting with Amanda, Chris, and Jim to discuss lab work
  • Team meeting

Tuesday 7/9/13

  • Meeting with Rouxi about using the Coulter Counter
  • HP meeting with Swetha, Betsy and Amanda

Wednesday 7/10/13

  • Meeting with exponent


Thursday 7/11/13

Friday 7/12/13

  • Austin’s HHMI presentation

Monday 7/15/13

  • Amanda meeting with Dr. Mosier about presentation to teachers
  • Dr. Chapell’s presentation on synthetic biology with aradopsis plants
  • Meeting with Dr. Clase about presentation to teachers

Tuesday 7/16/13

  • Mapped out timeline for HP activities

Wednesday 7/17/13

  • Transformation of cells
  • Amanda presentation of Modeling in AP Biology Classes

Thursday 7/18/13

Friday 7/19/13

  • Team meeting

Monday 7/22/13

  • Meeting with Amanda, Janie and Jim to discuss synthesis of BCDs
  • Cleaned out lab space
  • Meeting with Dr. Rickus and Amanda
  • Team meeting to discuss goals, lab rules, summer fundraising

Tuesday 7/23/13

Wednesday 7/24/13

Thursday 7/25/13

Friday 7/26/13

Monday 7/29/13

Tuesday 7/30/13

Wednesday 7/31/13

  • SURF presentations

Thursday 8/1/13

Friday 8/2/13