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Fungal secondary metabolites are an important source for the discovery of novel drugs due to their pharmacological properties. The production of secondary metabolites in most cases involves a big enzyme that contains multiple catalytic domains. We aim to use a synthetic approach by modulating each domain separately, and generate a plethora of new compounds that have a tremendous potential in saving human lives. The production of lovastatin has been chosen as an experimental concept. Our end product is non-replicative and contains no genetic material.

As we known, Synthetic Biology is an interesting and promising scientific field. Nonetheless, it is still subject to inherent hazards which may affect the researchers, the public and the environment. So it is crucially important for anyone working in this field is thoroughly aware of these dangers and takes the necessary precautions to minimize any risks that might occur. This page will provide an overview of the issues related to biological safety and security which is related to iGEM 2013 by providing answers to several key safety questions.

iGEM Safety Questions

1.Would any of your project ideas raise safety issues in terms of:

researcher safety,
public safety, or
environmental safety

No, our project ideas pose no substantial risk to either researchers, the public or the environment. In this project, all the organisms including E. coli and Aspergillus niger strains belong to Bio-safety level 1. They are non-pathogenic, commercially available and lab-safe. All members of our team have been trained in both Good Microbiological Techniques and general and specific lab safety. Besides, The existing biological parts we used don’t raise any foreseeable safety issues, so do the parts we introduced ourselves. All in all, (see Fungi-related safety).
More about General safety...

2.Do any of the new BioBrick parts (or devices) that you made this year raise safety issues? If yes,

Did you document these issues in the Registry?
How did you manage to handle the safety issue?
How could other teams learn from your experience?

No, none of the BioBrick parts or devices we made this year will raise safety issues. We produced secondary metabolites with industrial workhorse Aspergillus niger. Since it is known to be good at protein secretion, toleration of acidic environment and ‘generally regarded as safe’ (GRAS), Aspergillus niger is being introduced into iGEM as a novel chassis. Our main goal project is based on a multilevel approach by engineering parts, devices and our host. All of them are considered to be safe.
More about fungi-related safety...

3.Is there a local biosafety group, committee, or review board at your institution?

If yes, what does your local biosafety group think about your project?
If no, which specific biosafety rules or guidelines do you have to consider in your country?

Yes, Wageningen UR has its own biosafety rules next to the national biosafety regulations. Its rules can be found on laboratory safety page. Our laboratory and techniques all comply to the European Directive on the contained use of micro-organisms. Also it is approved by the Biosafety officer. All in all, the project is in compliance with national regulations and university requirements.
More about Biosafety Regulations...