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Team:Groningen/Labwork/27 August 2013
From 2013.igem.org
Inne
Did a mini prep of the colonies with biobrick BBa_k1085006, samples s7,s8,s11,s12,s13,s14. 30 uL elution buffer was used instead of 50 uL. Determined the concentration of the samples via nanodrop, also nanodropped the samples Sander miniprepped.| Sample | Concentration |
| s3 | 75.6 ng/L |
| s4 | 93.3 ng/L |
| s9 | 87.9 ng/L |
| s10 | 79.4 ng/L |
| s7 | 91.6 ng/L |
| s8 | 82.8 ng/L |
| s11 | 94.8 ng/L |
| s12 | 86.6 ng/L |
| s13 | 105.6 ng/L |
| s14 | 104.5 ng/L |
Inoculated the motility assay plate with wt, delta cheY and delta cheY/des. All strains will be grown in duplo overnight at 37C and 30C, time of inoculation: 18:20.
Sander
Did a mini prep of the colonies of samples s3,s4,s9,s10. Did a restriction digest of the samples and ran them over a 0,8% agarose gel. the samples were not present in the plasmids.Sebas
Designed and ordered primers to turn around the hy_spank promoter to end up with our final (correct) backbone:
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Found another backbone (pX), not biobrick compatible but with sites SpeI*SdaI (same overhang as XbaI*PstI).
iGEM 2013 Groningen
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