Team:Paris Bettencourt/Notebook/TB-ception/Thursday 29th August.html

From 2013.igem.org

Revision as of 15:10, 31 August 2013 by Ivaatanas (Talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

TB-ception

Thursday 1st August


We did a phagocytosis assay for our macrophages. First we passaged our cells and we determined their number (colored them with trypan blue and counted them): 2.68* 106, 3.72* 106 and 1.32* 106. To the first one we added 40, to the second 30 and to the last one 90 µl of NEB + pSB1A3 cell suspension. After 1 h we made this awesom picture:



Cloning from yesterday didn't work, so we repeated everything with longer digestion time for the backbone.

Retrieved from "http://2013.igem.org/Team:Paris_Bettencourt/Notebook/TB-ception/Thursday_29th_August.html"