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Exeter/29 August 2013
From 2013.igem.org
Revision as of 10:51, 2 September 2013 by JoshMiddleton (Talk | contribs)
Minipreps
We miniprepped:
- 1 + 4
- K592011 B
- K864404
- Cph8 #3
- 2 + 4
- K592022 B
- ENvZ no antibiotic
- ENvZ
- Cph8 #5
The liquid cultures of K592011 A and K592022 A did not grow.
NanoDrop from miniprep
| Part | Concentration (ng/ul) | |||
|---|---|---|---|---|
| K592011 B | 164.2 | |||
| B0015 | 37.7 | |||
| OmpR promoter | 50.7 | |||
| RBS | 49.1 |
| Part | OmpF (Part A) | B0034 (Part B) | AMP Plasmid | RFP (Control) |
|---|---|---|---|---|
| DNA | 12.4 ul | 9.4 ul | 10.0 ul | 5.0 ul |
| Non-nuclease Water | 3.6 ul | 6.6 ul | 5.5 ul | 11.0 ul |
| NEB Buffer | 2.5 ul | 2.5 ul | 2.5 ul | 2.5 ul |
| BSA | 0.5 ul | 0.5 ul | 0.5 ul | 0.5 ul |
| EcoR1 | 0.5 ul | - | 0.5 ul | 0.5 ul |
| Spe1 | 0.5 | - | - | - |
| Xbal1 | - | 0.5 ul | - | - |
| Pst1 | - | 0.5 ul | 0.5 ul | 0.5 ul |
| Dpn1 | - | - | 0.5 ul | - |
