29/08/13

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Contents

Make 2% agarose gel

Run Tod operon ligation genes on the gel

  • 1.5h at 70V

Isolate the DNA from gel

  • DNA isolated using Bioline Isolate II PCR and gel kit and following its protocol

Measuring concentration of DNA using nanodrop

SampleVolume ulConcentration ng/ul260/280260/230
A13014.71.811.41
A231131.711.43
B13023.61.931.02
B22921.91.881.33
C12914.71.830.63
C23015.51.860.64
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