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From 2013.igem.org

{ "date" : "2013-06-26", "author" : "emil-gabriele", "title" : "Ligation and transformation of R0010 + Sam sinthethase", "content" : "Today we added 1µl of SAP to the o/n digestion and then incubated it for 1.5h.Then we performed the ligation of SAMynthetase (previously digested with XbaI and PstI), following the ligation protocol.

Afterwards, Gabriele purified the product following the usual purification protocol and then transformed it in NEB10β cells following the transformation protocol.

Gabriele prepared also 4 inocula from the pSB1C3+R0010 CM-plate, just to be sure that it is (not) there.", "tags" : "Plac-SAMsynthetase" }