Exeter/5 August 2013

From 2013.igem.org

Exeter iGEM 2013 · Paint by Coli

Glycerol stocks

We made up 3 replicates of:

[http://parts.igem.org/Part:BBa_K592018 K592018]

[http://parts.igem.org/Part:BBa_S05058 S05058]

[http://parts.igem.org/Part:BBa_B0015 B0015]


Nano Drop

Culture NanoDrop concentration (ng/ul) size
K592018 160.7 2526bp
S05058 118.3 717bp
RFP 96.1 1047bp
B0015 76.3 129bp


Digestion

Part DNA (ul) dH20 (ul) Buffer (ul) BSA (ul) Enzyme 1 (ul) Enzyme 2 (ul) Enzyme 3 (ul)
K592018 1.6 14.4 2.5 0.5 EcoRI 0.5 SpeI 0.5 -
S05058 2.1 13.9 2.5 0.5 EcoRI 0.5 SpeI 0.5 -
B0015 3.3 12.7 2.5 0.5 XbaI 0.5 PstI 0.5 -
AMP plasmid 10 6 2.5 0.5 EcoRI 0.5 PstI 0.5 Dpnl 0.5
RFP 2.6 13.4 2.5 0.5 EcoRI 0.5 PstI 0.5 -

Ligation

Two methods using 'standard' amounts of each digest and using equimolar amounts.

Take me back to the notebook.

Exeter iGEM 2013 · Paint by Coli