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- Phage Purification
- March-April
- May-June
- July-August
- September-October
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7.3 EM Phage Preparation
I) Purpose
- Prepare phage so they can be viewed through an electron microscope.
II) Expected Outcome
- Visible phage with capsids and tails.
III) Reagants Used
- 300 mesh copper grid
- 2% phosphotungstic acid
- filter paper
- petri dish
- T4 lysate
- parafilm
IV) Actual Procedure
- Place parafilm in the petri dish and put a drop of T4 lysate and a drop of 2% phosphotungstic acid on the parafilm.
- Place the 300 grid mesh copper grid on the T4 lysate drop and leave for 20 minutes.
- Remove the grid and place on the 2% phosphotungstic acid drop and leave for 2 minutes.
- Remove the grid and dry with filter paper by lightly touching the tip to the grid.
- Place grid in the EM grid box.
V) Results
- The phage were prepared for an EM.
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