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Team:Paris Bettencourt/Notebook/Drug Screening/Saturday 21st September.html
From 2013.igem.org
Target
ASDFSaturday 21st September
Weekend Rush
Gibson assembly of pSB9S1, pSB10K1, and pSB3C7.
Digestion of sirA, fprA, fdxA and linearized backbone with XbaI and SpeI
Digestion of Linearized pSB1C3 with XbaI, SpeI, and DpnI
Ligation of sirA, fprA, and fdxA with pSB1C3
Heat Shock transformation of pSB1C3 sirA, pSB1C3 fprA and pSB1C3 fdxA into NEBTurbo
Heat Shock transformation of pSB9S1, pSB10K1 and pSB3C7 into NEBTurbo
