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From 2013.igem.org

Contents 1 Make 2% agarose gel 2 Run Tod operon ligation genes on the gel 3 Isolate the DNA from gel 4 Measuring concentration of DNA using nanodrop

Make 2% agarose gel

Run Tod operon ligation genes on the gel

• 1.5h at 70V

Isolate the DNA from gel

• DNA isolated using Bioline Isolate II PCR and gel kit and following its protocol

Measuring concentration of DNA using nanodrop

Sample	Volume ul	Concentration ng/ul	260/280	260/230
A1	30	14.7	1.81	1.41
A2	31	13	1.71	1.43
B1	30	23.6	1.93	1.02
B2	29	21.9	1.88	1.33
C1	29	14.7	1.83	0.63
C2	30	15.5	1.86	0.64