Team:Buenos Aires/ prueba

From 2013.igem.org

• Buenos aires
• Team
  • Students
  • Advisors
  • Instructors
• Project
  • Abstract
  • Motivation
  • The Device
• Model
  • Abstract
  • Motivation
  • The Device
• Results
  • mRFP Quantification
  • Response of GFP
  • Discenibility

Wetlab work at UBA labs

We chose for the chassis the cyanobacteria Synechocystis PCC 6803, because it is a photoautotroph and is regulated by circadian rhythms.

Using the lux operon and circadian promoters, we designed two plasmids: the Luciferase generator is to produce bioluminiscence, and the substrate generator recovers substainably the substrates necessary for the reactions.

We achieved to transform Synechocystis with the luciferase generator plasmid, and verificated light emission in a luminometer.

Al being lit by bioluminescent bacteria!

The Project

To Drink or Not To Drink

File:Howworks.gif